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Affinity Techniques
Analytical processes rely for their selectivity on the affinity between molecules,
which is the product of chemical attraction and shape complementarity. Apparently
difficult analyses can be transformed if a partner with high affinity for the analyte
can be identified. LGC had a success of this kind when it used a newly isolated
enzyme, gellan lyase, to digest the carbohydrate food additive, gellan gum, in order
to obtain fragments that could be resolved by capillary electrophoresis-mass
spectrometry (CE-MS)[1].
Other studies have developed our understanding of the use of antibody-antigen
reactions for the determination of small molecules presented in water-immiscible
organic solvents[2]; and for both immunoaffinity chromatography[3,4] and
immunoassay[4] of trace drugs from body fluids. We have adopted several approaches
to molecular imprinting, which is the use of analytes to mould previously unformed
materials into reusable selective binding partners. A working knowledge of
developments in chiral selectors is maintained so that flexible enantiomeric
separation methods can be rapidly implemented. We have developed expertise in
combinatorial approaches to the generation of DNA?based affinity ligands; and we have
scoped out the potential for combining affinity chemistry in situ with mass
spectrometry[5].
Bibliography
| 1. |
Craston D H, Farnell P, Francis J M, Gabriac S, Matthews W, Saeed M, and Sutherland I W, 'Determination of gellan gum by capillary electrophoresis and CE-MS'. Food Chem 2001, 73, 103-110.
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| 2. |
Francis J M and Craston D H, 'Immunoassay for parathion without its prior removal from solution in hexane'. Analyst 1994, 119, 1801-1805.
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| 3. |
Francis J M and Craston D H, 'Development of a stand-alone affinity clean-up for LSD in urine'. Analyst 1996, 121, 177-182.
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| 3. |
Webb K S, Baker P B, Cassells N P, Francis J M, Johnston D E, Lancaster S L, Minty P S, Reed G D, and White S A, 'The analysis of lysergide (LSD): the development of novel enzyme immunoassay and immunoaffinity extraction procedures together with an HPLC-MS confirmation procedure'. J Forensic Sci 1996, 41, 938-946.
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| 3. |
Francis J M, DTI Analytical Innovation programme: instrument evaluation: SELDI. Analytical Innovation web site: http://www.anamap.co.uk/, 2001.
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